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KMID : 0613820150250060624
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Journal of Life Science
2015 Volume.25 No. 6 p.624 ~ p.630
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Studies on Genetic Diversity and Phylogenetic relationships of Chikso (Korea Native Brindle Cattle) Using the Microsatellite Marker
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Choi Yeon-Ho
Seo Joo-Hee
Park Byoung-Ho
Lee Seung-Soo
Choi Tae-Jeong
Cho Kwang-Hyun
Choi Jae-Won
Jung Kyoung-Sub
Kong Hong-Sik
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Abstract
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This study examined the genetic distance among Chikso (Korea native brindle cattle) in nine regional areas using allele frequencies and a genetic diversity analysis with microsatellite markers. The analysis of the genetic diversity and genetic relationships of 2068 Chikso (383 KW, 180 GG, 52 KN, 129 KB, 332 UL, 24 JN, 198 JB, 148 CN, 622 CB) was carried out using 11 microsatellite markers. The number of alleles, observed heterozygostiy (Hobs), expected heterozygosity (Hexp), and polymorphism information content (PIC) of the 11 microsatellite markers were 8?24, 0.672?0.834, 0.687?0.886, and 0.638?0.876, respectively. The expected probability of identity values in random individuals (PI), random half-sib (PIhalf-sibs), and random sibs (PIsibs) were estimated to be 5.24×10-19, 2.63×10-06, and 2.63× 10-06, respectively, indicating that these markers can be used for traceability systems in Chikso cattle. The results of a phylogenetic tree (neighbor-joining tree), principle component analysis (PCA), and factorial component analysis (FCA) revealed genetic distance among nine Chikso populations. In conclusion, this study provides useful basic data that can be utilized in Chikso breeding and development. In addition, we will have to manage and conserve as a valuable genetic resource, without losing diversity of Chikso.
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KEYWORD
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Microsatellite, Chikso (Korea Native Brindle Cattle), Genetic diversity
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